NIH/3T3-Luc cell line

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NIH/3T3-Luc Order

NIH/3T3-Luc cell line
Catalog#
YC-A013-Luc-P
Size
1*10^6
Transfection Culture Medium
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
NIH/3T3-Luc cell line
Order

Detailed Product Information

Product category
Luciferase Stable Cell Lines
cell name
NIH/3T3-Luc
Cell Morphology
Fibroblast-like, adherent
Passage ratio
1:3~1:5
Species
Mouse
Reporter gene
Luciferase
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_C8Y9(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Luciferase validation
Sample Compound
pore 1
Compound
pore 2
Compound
pore 3
Average Expressed
multiple
NIH/3T3-Luc 158032 157843 185779 167218.0 182.022
NIH/3T3 952 987 817 918.667
Loci STR Info (Sample Cell)
Sample Cell Line:NIH/3T3
STR Info (Cell bank)
Cell Line:NIH 3T3
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.019.017.019.0
4-2 19.320.319.320.3
6-7 12.012.0
19-2 10.011.012.011.012.0
1-2 13.017.013.017.0
7-1 29.029.0
8-1 15.015.0
1-1 10.010.0
3-2 14.015.014.015.0
2-1 9.09.0
15-3 20.320.3
6-4 16.015.3
13-1 16.216.2
11-2 15.017.015.017.0
17-2 13.014.013.014.0
12-1 20.020.0
5-5 14.015.014.015.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the NIH/3T3 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NIH/3T3-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

  • Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
    01
  • Sensitive accurate:Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
    02
  • High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
    03
NIH/3T3-Luc cell line
NIH/3T3-Luc
Catalog#
YC-A013-Luc-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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